ngwaahịa

Chloramphenicol bụ ọgwụ nje dị iche iche na-arụ ọrụ nke ọma, nke a na-ejikarị agwọ ọrịa anụmanụ dị iche iche na-efe efe, ma nwee mmetụta mgbochi na ụdị nje bacteria dị iche iche.Nsogbu siri ike na chloramphenicol residues.Chloramphenicol nwere mmetụta dị njọ na-egbu egbu na mmetụta dị n'akụkụ, nke nwere ike igbochi ọrụ hematopoietic nke ụmị ọkpụkpụ mmadụ, na-eme ka anaemia nke mmadụ, granular leukocytosis, neonatal, ọrịa isi awọ na-akaghị aka na ọrịa ndị ọzọ, na obere mkpokọta nke ọgwụ fọdụrụnụ nwekwara ike ịkpata ọrịa.Ya mere, chloramphenicol residues na nri anụmanụ na-etinye nnukwu ihe iyi egwu na ahụike mmadụ.Ya mere, amachibidoro ya ma ọ bụ jiri ya mee ihe na EU na US.

Kwinbon ngwa a bụ ngwaahịa ọhụrụ dabere na ELISA, nke dị ngwa ngwa (naanị 50min n'otu ọrụ), dị mfe, nke ziri ezi na nke nwere mmetụta ma e jiri ya tụnyere nyocha ngwa ngwa, yabụ na ọ nwere ike belata nke ukwuu njehie ọrụ yana ike ọrụ.

Mmeghachi omume

Chloramphenicol………………………………………………………………………………………………………………

Chloramphenicol palmitate………………………………………………………………………….

Thiamphenicol………………………………………………………………………………………………………….

Florfenicol………………………………………………………………………………………………………………………………………………

Cetofenicol………………………………………………………………………………………………………………………

Ngwa ngwa

Efere Microtiter nke ejiri antigen kpuchie, 96wells

Ihe ngwọta ọkọlọtọ (6 × 1ml / karama)

0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb

Ngwọta ọkọlọtọ na-agbapụta: (1ml / karama) ……..100pb

Concentrated enzyme conjugate 1ml …………………………………………………………………………………………………..

Enzyme conjugate diluent 10ml …………………………………………………………………………………………………….

Ngwọta A 7ml ………………………………………………………… …………………………………………. okpu ọcha

Ngwọta B 7ml ………………………………………………………………… .......................................... okpu uhie

Kwụsị ngwọta 7ml ………………………………………………………… ………………………………….. okpu edo edo

20× Ngwọta ịsa ahụ gbadoro anya 40ml………………………………………………………………. transperent okpu

2 × Ngwọta mmịpụta etinyere 50ml............................................ ...........Okpu na-acha anụnụ anụnụ

Nsonaazụ

1 pasent nnabata

A na-ekewa ụkpụrụ nke ụkpụrụ nnabata nke enwetara maka ụkpụrụ na ihe nlele site na uru nnabata nke ọkọlọtọ mbụ (ọkọlọtọ efu) ma mụbaa site na 100%.A na-eme ọkọlọtọ efu ka ọ bụrụ 100% yana ụkpụrụ nnabata na-ehota na pasentị.

B ——ụkpụrụ nnabata (ma ọ bụ sample)

B₀ ——ụkpụrụ efu efu

2 Usoro ọkọlọtọ

Iji see usoro ọkọlọtọ: were uru nnabata nke ụkpụrụ dị ka y-axis, ọkara logarithmic nke ntinye uche nke ụkpụrụ ụkpụrụ CAP (ppb) dị ka axis x.

A na-amụba ntinye uche CAP nke ihe nlele ọ bụla (ppb), nke enwere ike ịgụ site na nhazi nhazi, site na ihe Dilution nke kwekọrọ na nke ọ bụla na-esote, a na-enwetakwa ntinye uche n'ezie.

Biko mara:

Maka nyocha data nke ngwa ELISA, emepụtara sọftụwia pụrụ iche, nke enwere ike inye iwu na arịrịọ.