umkhiqizo

I-Chloramphenicol iyi-antibiotic esebenzayo ebanzi, evame ukusetshenziswa ekwelapheni izifo ezahlukahlukene ezithathelwanayo zezilwane, futhi inomphumela ovimbelayo ezinhlobonhlobo zamabhaktheriya e-pathogenic.Inkinga enkulu ngezinsalela ze-chloramphenicol.I-Chloramphenicol inobuthi obuyingozi kanye nemiphumela engemihle, engavimbela ukusebenza kwe-hematopoietic yomnkantsha womuntu, ibangele i-anemia ye-aplastic yomuntu, i-leukocytosis eyigranular, i-neonatal, i-gray syndrome ngaphambi kwesikhathi kanye nezinye izifo, futhi ukugxila okuphansi kwezinsalela zezidakamizwa nakho kungabangela izifo.Ngakho-ke, izinsalela ze-chloramphenicol ekudleni kwezilwane ziyingozi enkulu empilweni yabantu.Ngakho-ke, ivinjelwe noma isetshenziswa ngokuqinile e-EU nase-US.

I-Kwinbon le khithi iwumkhiqizo omusha osuselwe ku-ELISA, eshesha (imizuzu engama-50 kuphela ekusebenzeni okukodwa), elula, enembile futhi ebucayi uma iqhathaniswa nokuhlaziywa kwezinsimbi ezivamile, ngakho inganciphisa kakhulu iphutha lokusebenza kanye nokuqina komsebenzi.

Ukusabela okuphambene

I-Chloramphenicol ………………………………………………… 100%

I-Chloramphenicol palmitate………………………………<0.1%

I-Thiamphenicol…………………………………..……..<0.1%

I-Flofenicol…………………………………………………<0.1%

I-Cetofenicol…………………………………………………<0.1%

Izingxenye Zekhithi

Ipuleti le-Microtiter elimbozwe nge-antigen, ama-96wells

Izixazululo ezijwayelekile (6×1ml/ibhodlela)

0ppb, 0.025ppb, 0.075ppb, 0.3ppb, 1.2ppb, 4.8ppb

Isixazululo esijwayelekile se-spiking: (1ml/ibhodlela) …….…100ppb

I-enzyme egxilile i-conjugate 1ml….…..……………..….…..….…..….………….

I-enzyme conjugate diluent 10ml….…..….………………….…..….…..….…..…..ikepisi esobala

Isixazululo A 7ml …………………………………………… ..............….………….ikepisi elimhlophe

Isixazululo B 7ml …………………………………………………… .........……………….ikepisi elibomvu

Isixazululo sokumisa 7ml ………………………………………………… ............…….…….…..ikepisi eliphuzi

20×Isixazululo sokugeza esigxilisiwe 40ml………………………………………….….ikepisi esobala

2×Isixazululo sokukhipha okugxilile 50ml............................................ ...........ikepisi eliluhlaza okwesibhakabhaka

Imiphumela

1 Iphesenti lokumunca

Amanani amaphakathi wamanani okumunca atholwe kumazinga kanye namasampuli ahlukaniswa ivelu yokumunca yezinga lokuqala (izinga elinguziro ) futhi liphindwe ngo-100%.Izinga elinguziro lenziwa lalingana no-100% futhi amanani okumunca acashunwe ngamaphesenti.

B ——izinga le-absorbance (noma isampula)

B₀ ——absorbance zero standard

2 Ijika Elijwayelekile

Ukuze udwebe ijika elijwayelekile: thatha inani lokumunca lamazinga njenge-y-eksisi, i-semi logarithmic yokugxiliswa kwesisombululo sezindinganiso ze-CAP (ppb) njenge-x-eksisi.

Ukugxiliswa kwe-CAP kwesampula ngayinye (ppb), engafundwa kwijika lokulinganisa, kuphindwa nge-Dilution factor ehambisanayo yesampula ngayinye elandelwayo, futhi ukugxiliswa kwangempela kwesampula kuyatholakala.

Sicela uqaphele:

Ukuze kuhlaziywe idatha ye-ELISA kits, isofthiwe ekhethekile iye yathuthukiswa, enga-odwa ngesicelo.