ʻO Elisa hoʻāʻo pahu o CAP
ʻO Chloramphenicol kahi lāʻau antibiotic ākea ākea, hoʻohana mau ʻia i ka mālama ʻana i nā maʻi lele like ʻole o nā holoholona, a he hopena pale i nā ʻano bacteria pathogenic.ʻO ka pilikia nui me nā koena chloramphenicol.He koʻikoʻi ko Chloramphenicol a me nā hopena ʻaoʻao, hiki ke pale i ka hana hematopoietic o ka iwi iwi iwi, e hoʻoulu ai i ka anemia aplastic kanaka, granular leukocytosis, neonatal, premature gray syndrome a me nā maʻi ʻē aʻe, a me nā haʻahaʻa haʻahaʻa o nā koena lāʻau hiki ke hoʻoulu i ka maʻi.No laila, ʻo ke koena o ka chloramphenicol i ka meaʻai holoholona he mea weliweli nui i ke olakino kanaka.No laila, ua pāpā ʻia a hoʻohana paʻa ʻia ma EU a me US.
He huahana hou kēia kit ma muli o ELISA, ʻo ia ka wikiwiki (50 mau minuke wale nō i ka hana hoʻokahi), maʻalahi, pololei a maʻalahi hoʻi i ka hoʻohālikelike ʻia me ka loiloi instrumental maʻamau, a no laila hiki iā ia ke hōʻemi nui i ka hewa o ka hana a me ka ikaika o ka hana.
Nā hoʻololi keʻa
Chloramphenicol………………………………………… 100%
Chloramphenicol palmitate……………………………………<0.1%
Thiamphenicol…………………………………………..<0.1%
Florfenicol………………………………………………<0.1%
Cetofenicol…………………………………………………………<0.1%
Nā ʻāpana pahu
ʻO ka pā Microtiter i uhi ʻia me ka antigen, 96wells
Nā hāʻina maʻamau (6×1ml/ʻōmole)
0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb
ʻO ka hoʻonā maʻamau maʻamau: (1ml/bottle) …….…100ppb
Hoʻohui ʻia ka enzyme conjugate 1ml…………..………..………..……….…….
Enzyme conjugate diluent 10ml…………………………………………………………………………………………………………….
Hoʻonā A 7ml…………………………………………………… ...........……………….pale keʻokeʻo
Pane B 7ml…………………………………………………… .....................pale ʻulaʻula
Hoʻopau i ka hoʻonā 7ml…………………………………………………… ......................palemele melemele
20×Hoʻonā holoi holoi paʻa 40ml………………………………………………….pale mālohi
2×Hui hoʻoheheʻe ʻia 50ml................................................ ........... pāpale polū
Nā hualoaʻa
1 Pākēneka o ke komo ʻana
Hoʻokaʻawale ʻia nā kumukūʻai kumu o nā waiwai absorbance i loaʻa no nā maʻamau a me nā laʻana e ka waiwai absorbance o ka maʻamau mua (zero standard) a hoʻonui ʻia e 100%.No laila, ua like ka zero standard me 100% a ua helu ʻia nā helu absorbance ma nā pākēneka.
B ——absorbance maʻamau (a i ʻole ka laʻana)
B0 ——absorbance zero standard
2 Pipi maʻamau
No ke kaha kiʻi maʻamau: e lawe i ka waiwai hoʻopaneʻe o nā maʻamau ma ke ʻano he axis y, semi logarithmic o ka neʻe ʻana o ka hopena maʻamau CAP (ppb) ma ke ʻano he x-axis.
Ua hoonuiia ka manao CAP o kela a me keia lapana (ppb), i hiki ke heluheluia mai ke kaha o ka calibration, e ka helu Dilution like o kela me keia la'ana i ukaliia, a ua loaa ka manao maoli o ka laana.
E ʻoluʻolu e hoʻomaopopo:
No ka ʻikepili ʻikepili o nā kits ELISA, ua hoʻomohala ʻia nā polokalamu kūikawā, hiki ke kauoha ʻia ma ke noi.
