hua

Ko te Chloramphenicol he paturopi-a-whanui whai hua, e whakamahia nuitia ana ki te rongoa i nga momo mate hopuhopu o nga kararehe, me te aukati i nga momo huakita pathogenic.He raru nui ki nga toenga chloramphenicol.Ko te Chloramphenicol he tino paitini me nga paanga taha, ka taea te aukati i te mahi hematopoietic o te wheua wheua tangata, ka puta te anemia aplastic tangata, te leukocytosis granular, te neonatal, te mate hina o mua me etahi atu mate, me te iti o nga toenga o te tarukino ka taea hoki te mate.Na reira, ko nga toenga o te chloramphenicol i roto i nga kai kararehe he tino whakatuma ki te hauora o te tangata.Na reira, kua whakakorehia, kua whakamahia ranei i roto i te EU me te US.

Ko Kwinbon tenei kete he hua hou i runga i te ELISA, he tere (50 meneti anake i roto i te mahi kotahi), he ngawari, he tika me te tairongo ki te whakataurite ki te tātari taputapu noa, na reira ka taea te whakaiti i te hapa mahi me te kaha o te mahi.

Whakawhitiwhiti

Chloramphenicol……………………………………………………100%

Chloramphenicol palmitate……………………………………<0.1%

Thiamphenicol……………………………………………………..<0.1%

Florfenicol………………………………………………<0.1%

Cetofenicol…………………………………………………………<0.1%

Nga Waahanga Kete

Pereti Microtiter kua pania ki te antigen, 96wells

Nga otinga paerewa (6×1ml/pounamu)

0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb

Te otinga paerewa mokowhiti: (1ml/pounamu) …….…100ppb

Whakakotahi pūmua whākōkī 1ml…………………………………………………………………………………………………

Whakawhanake whakahuihui enzyme 10ml……………………………………………………………………………………………………………..puata marama

Otinga A 7ml…………………………………………………… .............................potae ma

Otinga B 7ml……………………………………………………………… ...............potae whero

Whakamutua te otinga 7ml…………………………………………………… ...........……...potae kōwhai

20×Warewa horoi kukū 40ml……………………………………………..potae maramara

2×Te otinga tangohanga 50ml............................................ ..........potae kahurangi

Hua

1 Ōrau te horomitanga

Ko nga uara toharite o nga uara urunga kua whiwhi mo nga paerewa me nga tauira ka wehewehea e te uara whakauru o te paerewa tuatahi (kore paerewa) ka whakareatia ki te 100%.Ko te paerewa kore ka hangaia kia rite ki te 100% ka whakahuahia nga uara urunga i roto i nga ōrau.

B ——paerewa horonga (tauira ranei)

B₀ ——absorbance zero standard

2 Anau Paerewa

Hei tuhi i te anau paerewa: tangohia te uara ngongo o nga paerewa hei tuaka-y, hauruarua o te kukū o te otinga paerewa CAP (ppb) hei tuaka-x.

Ko te kukū o te CAP o ia tauira (ppb), ka taea te panui mai i te ananga whakatikatika, ka whakareatia ki te tauwehe Dilution e rite ana o ia tauira ka whai, ka whiwhi i te tino kukū o te tauira.

Kia mahara:

Mo te tātaritanga raraunga o nga kete ELISA, kua whakawhanakehia he rorohiko motuhake, ka taea te tono i runga i te tono.