mankhwala

Chloramphenicol ndi mankhwala ophatikizika kwambiri, omwe amagwiritsidwa ntchito pochiza matenda osiyanasiyana a nyama, ndipo amalepheretsa mabakiteriya osiyanasiyana.Vuto lalikulu ndi zotsalira za chloramphenicol.Chloramphenicol ali kwambiri poizoni ndi mavuto, amene angalepheretse hematopoietic ntchito ya m`mafupa anthu, kuchititsa anthu aplastic magazi m`thupi, granular leukocytosis, neonatal, msanga imvi syndrome ndi matenda ena, ndi otsika woipa wa zotsalira za mankhwala kungachititsenso matenda.Chifukwa chake, zotsalira za chloramphenicol muzakudya za nyama ndizowopsa kwambiri ku thanzi la munthu.Chifukwa chake, idaletsedwa kapena kugwiritsidwa ntchito mochepera ku EU ndi US.

Kwinbon chida ichi ndi chinthu chatsopano chochokera ku ELISA, chomwe chimakhala chachangu (50min kokha pakugwira ntchito imodzi), yosavuta, yolondola komanso yachidziwitso poyerekeza ndi kusanthula kwa zida zodziwika bwino, motero imatha kuchepetsa kulakwitsa kwa magwiridwe antchito komanso kuchuluka kwa ntchito.

Zosiyanasiyana

Chloramphenicol ……………………………………………… 100%

Chloramphenicol palmitate ………………………………<0.1%

Thiamphenicol ……………………………………………..<0.1%

Florfenicol …………………………………………………<0.1%

Cetofenicol…………………………………………………<0.1%

Zida Zamagetsi

Mbale ya Microtiter yokutidwa ndi antigen, 96wells

Njira zokhazikika (6×1ml/botolo)

0ppb, 0.025ppb, 0.075ppb, 0.3ppb, 1.2ppb, 4.8ppb

Spiking standard solution: (1ml/botolo) …….…100ppb

Concentrated enzyme conjugate 1ml….…..………………..….…..….…..….…… kapu yowonekera

Enzyme conjugate diluent 10ml….…..………………………………………..….…..….…..…..transparent cap

Solution A 7ml ……………………………………… ..............….………….kapu woyera

Solution B 7ml ……………………………………………… .........……………….kapu yofiyira

Stop solution 7ml ………………………………………… ........…….…….…..yellow cap

20×Wash solution 40ml………………………………………….….kapu yowonekera

2 × Anayikira m'zigawo njira 50ml .......................................... ...........kapu yabuluu

Zotsatira

1 peresenti ya absorbance

Ziwerengero zamtengo wapatali zazitsulo zomwe zimatengedwa pamiyeso ndi zitsanzo zimagawidwa ndi kulowetsedwa kwa mlingo woyamba (zero standard) ndikuchulukitsa ndi 100%.Muyezo wa zero umapangidwa kukhala wofanana ndi 100% ndipo milingo ya kuyamwa imatchulidwa mwamaperesenti.

B --absorbance muyezo (kapena chitsanzo)

B₀ ——absorbance zero standard

2 Mapindikira Okhazikika

Kuti mujambule mapindikidwe okhazikika: tengani kuchuluka kwa milingo ngati y-axis, semi logarithmic ya ndende ya CAP standards solution (ppb) ngati x-axis.

Kuphatikizika kwa CAP kwachitsanzo chilichonse (ppb), chomwe chitha kuwerengedwa kuchokera pamapindikira owongolera, kumachulukitsidwa ndi gawo lofananira la Dilution lachitsanzo chilichonse chotsatiridwa, ndipo kuchuluka kwenikweni kwa zitsanzo kumapezedwa.

Chonde zindikirani:

Kuti mudziwe zambiri za zida za ELISA, mapulogalamu apadera apangidwa, omwe angathe kuyitanidwa popempha.