ibicuruzwa

Umubare munini wa aflatoxine utera uburozi bukabije (aflatoxicose) bushobora guhitana ubuzima, akenshi binyuze mu kwangiza umwijima.
Aflatoxin B1 ni aflatoxine ikorwa na Aspergillus flavus na A. parasitike.Ni kanseri ikomeye cyane.Izi mbaraga za kanseri ziratandukanye bitewe nubwoko bumwe na bumwe, nk'imbeba n'inguge, bisa nkaho byoroshye kurusha ibindi.Aflatoxin B1 ni umwanda ukabije mu biribwa bitandukanye birimo ibishyimbo, ifunguro ry'imbuto, ibigori, n'ibindi binyampeke;kimwe no kugaburira amatungo.Aflatoxin B1 ifatwa nka aflatoxine ifite ubumara bukabije kandi ifite uruhare runini muri kanseri ya hepatocellular kanseri (HCC) mu bantu.Uburyo bwinshi bwo gutoranya no gusesengura harimo chromatografi yoroheje (TLC), chromatografiya ikora cyane (HPLC), mass spectrometrie, hamwe na enzyme ihujwe na immunosorbent assay (ELISA), nibindi, byakoreshejwe mugupima kwanduza aflatoxine B1 mubiribwa .Ishami ry’ibiribwa n’ubuhinzi (FAO) rivuga ko ku isi hose urugero rwa aflatoxine B1 rwihanganirwa ku isi hose ruri hagati ya 1–20 μg / kg mu biribwa, na 5-50 μg / kg mu biryo by’inka by’ibiryo mu 2003.

Ibisobanuro

1.Elisa Ikizamini cya Aflatoxin B1

2.Cat.KA07202H-96 Amazu

3. Ibigize ibikoresho
● Microtiter yashizwemo antigen, amariba 96
Solution Igisubizo gisanzwe × 6 Icupa (1ml / icupa)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml ……………………………………………………… .. ………… umutuku
Solution Igisubizo cya Antibody7ml ............................................... ................................... ……… ingofero yicyatsi
Gukuramo A 7ml …………………………………………………………. ………… ... cap cap cap
Substrate B 7ml ………………. ………………………………………………. ………… umutuku
Hagarika igisubizo 7ml ………. ……. ……………………………………………… .. ……… cap yumuhondo
● 20 × yibanze yo gukaraba 40ml ………………………………………… capa ibonerana
● 2 × igisubizo cyo gukuramo 50ml ……………………………………………… cap yubururu

4.Ibyiyumvo, ukuri kandi neza
Ibyiyumvo: 0.05ppb

5.Imipaka ntarengwa
Icyitegererezo cyamavuta yo kurya ............................................... .................................................. ........................ 0.1ppb
Ibishyimbo ................................................. .................................................. ....................... 0.2ppb
Ibinyampeke ................................................. .................................................. ...................... 0.05ppb
Ukuri
Icyitegererezo cyamavuta yo kurya ............................................... .................................................. .................... 80 ± 15%
Ibishyimbo ................................................. .................................................. ..................... 80 ± 15%
Ibinyampeke ................................................. .................................................. ..................... 80 ± 15%
Icyitonderwa:Coefficient yo gutandukanya ibikoresho bya ELISA iri munsi ya 10%.

6.Igipimo rusange
Aflatoxin B1 ·················· 100%
Aflatoxin B2 ····················.
Aflatoxin G1 ··················· 62%
Aflatoxin G2 ··················.