Elisa Test Kit ya Aflatoxin B1
Dozi kubwa za aflatoxins husababisha sumu kali (aflatoxicosis) ambayo inaweza kutishia maisha, kwa kawaida kupitia uharibifu kwenye ini.
Aflatoxin B1 ni aflatoxin inayozalishwa na Aspergillus flavus na A. parasiticus.Ni kasinojeni yenye nguvu sana.Uwezo huu wa kusababisha saratani hutofautiana kati ya spishi na baadhi, kama vile panya na nyani, wanaonekana kushambuliwa zaidi kuliko wengine.Aflatoxin B1 ni uchafuzi wa kawaida katika vyakula mbalimbali ikiwa ni pamoja na karanga, unga wa pamba, mahindi, na nafaka nyinginezo;pamoja na vyakula vya mifugo.Aflatoksini B1 inachukuliwa kuwa aflatoksini yenye sumu zaidi na inahusishwa sana na saratani ya hepatocellular (HCC) kwa binadamu.[inahitajika] Kwa wanyama, aflatoksini B1 pia imeonyeshwa kuwa mutajeni, teratogenic, na kusababisha ukandamizaji wa kinga mwilini.Mbinu kadhaa za sampuli na uchanganuzi ikijumuisha kromatografia ya safu-nyembamba (TLC), kromatografia ya utendakazi wa hali ya juu ya kioevu (HPLC), spectrometry ya wingi, na kipimo cha immunosorbent kilichounganishwa na enzyme (ELISA), miongoni mwa zingine, zimetumika kupima uchafuzi wa aflatoxin B1 katika vyakula. .Kulingana na Shirika la Chakula na Kilimo (FAO), viwango vya juu zaidi vya kuvumiliwa vya aflatoxin B1 ulimwenguni viliripotiwa kuwa kati ya 1-20 μg/kg katika chakula, na 5-50 μg/kg katika malisho ya ng’ombe mnamo 2003.
Maelezo
1.Elisa Test Kit kwa Aflatoxin B1
2.Paka.KA07202H-96wells
3. Vipengele vya kit
● Microtiter plateprecoated na antijeni, 96 visima
● Suluhisho la Kawaida ×6chupa(1ml/chupa)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml……………………………………………………………..………… kofia nyekundu
● Suluhisho la kingamwili7ml............................................. ................................................................. kofia ya kijani
● Substrate A 7ml …………………………………………………………………………………… kofia nyeupe
● Substrate B 7ml……………….…………………………………………………….…………… kofia nyekundu
● Suluhisho la kuacha 7ml……….…….……………………………………………………..……… kofia ya njano
● 20× suluhisho la kuosha lililokolea 40ml …………………………………………… kofia ya uwazi
● 2×suluhisho la uchimbaji lililokolea 50ml…………………………………………………
4.Usikivu, usahihi na usahihi
Unyeti: 0.05ppb
5.Kikomo cha kugundua
Sampuli ya mafuta ya kula.......................................... .................................................. .......................0.1ppb
Karanga................................................. .................................................. .......................0.2ppb
Nafaka................................................. .................................................. .....................0.05ppb
Usahihi
Sampuli ya mafuta ya kula.......................................... .................................................. .................80±15%
Karanga................................................. .................................................. .....................80±15%
Nafaka................................................. .................................................. .....................80±15%
Usahihi:Mgawo wa tofauti wa kit ELISA ni chini ya 10%.
6.Kiwango cha Msalaba
Aflatoxin B1 ··················100%
Aflatoxin B2 ·······················81 .3%
Aflatoxin G1 ·······················62%
Aflatoxin G2 ·······················22.3%
