chigadzirwa

About

Iyi kit inogona kushandiswa muhuwandu uye hwemhando yekuongorora kweAMOZ zvakasara muzvigadzirwa zvemvura (hove uye shrimp), nezvimwewo. Enzyme immunoassays, kana ichienzaniswa nechromatographic nzira, inoratidza zvakanakira kunzwisisika, muganhu wekuona, michina yehunyanzvi uye nguva inodiwa.

Iyi kit yakagadzirirwa kuona AMOZ zvichibva pamusimboti weiyo indirect yemakwikwi enzyme immunoassay.Iyo microtiter matsime akaputirwa nekubata BSA yakabatana

antigen.AMOZ mumuenzaniso inokwikwidza neantigen yakavharwa pa microtiter plate yeantibody yakawedzerwa.Mushure mekuwedzera kwe enzyme conjugate, chromogenic substrate inoshandiswa uye chiratidzo chinoyerwa ne spectrophotometer.Kunyura kwacho kunopesana ne AM OZ concentration mumuenzaniso.

Kit Zvikamu

·Microtiter plate ine matsime makumi mapfumbamwe nematanhatu akaputirwa neantigen

· Yakajairwa mhinduro (6 mabhodhoro)

0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb

·Spiking standard solution: (1ml/bhodhoro) …………………………………………………100ppb

Enzyme conjugate 1ml…………………………………………………………..…………….

·Antibody solution 7ml ……………………………………………………..………….….green cap

·solution A 7ml………………………………………………………….…………………kepisi chena

·solution B 7ml……………………………………………………………….………..…… chivharo chitsvuku

·stop solution 7ml ………………………………………………………….…………yellow cap

·20×concentrated wash solution 40ml……………………………………….……

·2×concentrated extraction solution 50ml………………………………………….….

·2-Nitrobenzaldehyde 15.1mg…………………………………………………………….

Sensitivity, kururama uye nemazvo

Sensitivity: 0.05ppb

Detection limit

Zvigadzirwa zvemumvura(hove uye shrimp)…………………… 0.1ppb

Kururama

Zvigadzirwa zvemumvura(hove nema shrimp)…………………… 95±25%

Precision:CV ye ELISA kit iri pasi pe10%.

Cross Rate

Furaltadone metabolite(AMOZ)…………………………………….…………100%

Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%

Nitrofurantoin metabolite(AHD)………………………………….…………<0.1%

Nitrofurazone metabolite(SEM)……………………………………………..…<0.1%

Furaltadone………………………………………………………………….…….11.1%

Furazolidone ………………………………………………………….…...…<0.1%

Nitrofurantoin………………………………………………………….….…<1%

Nitrofurazone………………………………………………….………………..…<1%