samfur

Game da

Ana iya amfani da wannan kit ɗin a cikin ƙididdigewa da ƙididdige ƙididdiga na ragowar AMOZ a cikin samfuran ruwa (kifi da shrimp), da sauransu. Enzyme immunoassays, idan aka kwatanta da hanyoyin chromatographic, yana nuna fa'idodi masu yawa game da hankali, iyakokin ganowa, kayan fasaha da buƙatun lokaci.

An tsara wannan kit ɗin don gano AMOZ bisa ƙa'idar gasa ta immunoassay na enzyme kai tsaye.An lulluɓe rijiyoyin microtiter tare da haɗin BSA kama

antigen.AMOZ a cikin samfurin yana gasa da antigen da aka lulluɓe akan farantin microtiter don ƙarar rigakafin.Bayan ƙari na haɗin enzyme , ana amfani da substrate na chromogenic kuma ana auna siginar ta hanyar spectrophotometer.Abun sha ya yi daidai da daidaitawar AM OZ a cikin samfurin.

Abubuwan Kit

·Microtiter plate mai rijiyoyi 96 da aka lullube da antigen

Daidaitaccen mafita (kwalabe 6)

0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb

· Madaidaicin bayani: (1ml/kwalba) …………………………………………………………………………100ppb

Enzyme conjugate 1ml…………………………………………………………………………………………. ja hula

Maganin antibody 7ml ……………………………………………………………………………………. koren hula

Magani A 7ml……………………………………………………………………………………….…………………. farar hula

Magani B 7ml……………………………………………………………………………………….………………. jan hula

Magani tasha 7ml………………………………………………………………………………............ rawaya hula

· 20× mayar da hankali wanke bayani 40ml……………………………………………………………. m hula

· 2× mayar da hankali hakar bayani 50ml…………………………………………………………………. shuɗi hula

2-Nitrobenzaldehyde 15.1mg………………………………………………………………………………………………

Hankali, daidaito da daidaito

Hankali: 0.05ppb

Iyakar ganowa

Kayayyakin ruwa (kifi da jatan lande)……………………………… 0.1ppb

Daidaito

Kayayyakin ruwa (kifi da jatan lande)………………………… 95± 25%

Daidaito:CV na kit ɗin ELISA bai wuce 10% ba.

Tsare-tsare

Furaltadone metabolite (AMOZ).………… 100%

Furazolidone metabolite (AMOZ)..………………………….<0.1%

Nitrofurantoin metabolite (AHD).………………… <0.1%

Nitrofurazone metabolite (SEM) ………………………………………………………………….... <0.1%

Furaltadone………………………………………………………………………………………….……..11.1%

Furazolidone …………………………………………………………........ <0.1%

Nitrofutantoin .....................

Nitrofurazone ………………………………………………………….…………………………. <1%