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Apo Idanwo Elisa ti AMOZ Ifihan Aworan
  • Elisa Idanwo AMOZ
  • Elisa Idanwo AMOZ
  • Elisa Idanwo AMOZ
  • Elisa Idanwo AMOZ

Elisa Idanwo AMOZ

Apejuwe kukuru:

Awọn oogun nitrofuran furaltadone, nitrofurantoin ati nitrofurazone ti ni idinamọ lati lo ninu iṣelọpọ ẹran ounjẹ ni EU ni 1993, ati lilo furazolidone ti ni idinamọ ni ọdun 1995. Ayẹwo ti awọn iyokù oogun nitrofuran nilo lati da lori wiwa ti awọn metabolites ti o ni asopọ tissu ti awọn oogun obi nitrofuran, niwọn igba ti awọn oogun obi ti ni iṣelọpọ ni iyara pupọ, ati pe awọn metabolites nitrofuran ti a so pọ yoo da duro fun igba pipẹ, nitorinaa a lo awọn metabolites bi ibi-afẹde ni wiwa ilokulo ti nitrofurans.Furazolidone metabolite (AMOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) ati Nitrofurazone metabolite (SEM).

Ologbo.KA00205H-96 kanga


Awọn Itọsọna Fun Lilo

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Nipa

Ohun elo yii le ṣee lo ni pipo ati igbekale agbara ti aloku AMOZ ni awọn ọja omi (ẹja ati ede), bbl Awọn ajẹsara Enzyme, ni afiwe pẹlu awọn ọna chromatographic, ṣafihan awọn anfani nla nipa ifamọ, opin wiwa, ohun elo imọ-ẹrọ ati ibeere akoko.

Ohun elo yii jẹ apẹrẹ lati ṣe awari AMOZ ti o da lori ipilẹ ti ajẹsara ajẹsara enzymu ifigagbaga aiṣe-taara.Awọn kanga microtiter ti wa ni ti a bo pẹlu Yaworan BSA ti sopọ

antijeni.AMOZ ni apẹẹrẹ ti njijadu pẹlu antijeni ti a bo lori awo microtiter fun agboguntaisan ti a ṣafikun.Lẹhin afikun ti enzymu conjugate, a lo sobusitireti chromogenic ati pe ifihan naa jẹ iwọn nipasẹ spectrophotometer kan.Imudani jẹ iwọn idakeji si ifọkansi AM OZ ninu apẹẹrẹ.

Ohun elo Kit

· Microtiter awo pẹlu kanga 96 ti a bo pẹlu antijeni

Awọn ojutu boṣewa (awọn igo 6)

0ppb, 0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb

Ojutu boṣewa spiking: (1ml/igo) …………………………………………………………………………100ppb

Enzyme conjugate 1ml………………………………………………………………………………………. fila pupa

· Ojutu antibody 7ml …………………………………………………………………………………. fila alawọ ewe

ojutu A 7ml……………………………………………………………………………….………………… fila funfun

ojutu B 7ml……………………………………………………………………………………….………………………. fila pupa

ojutu iduro 7ml ………………………………………………………………………………….………… fila ofeefee

· 20×Ojutu fifọ ogidi 40ml……………………………………………………… fila sihin

· 2× ojutu isediwon ogidi 50ml………………………………………………………………. fila buluu

2-Nitrobenzaldehyde 15.1mg……………………………………………………………………………………………….

Ifamọ, išedede ati konge

Ifamọ: 0.05ppb

Iwọn wiwa

Awọn ọja omi (ẹja ati ede) ………………………… 0.1ppb

Yiye

Awọn ọja inu omi (ẹja ati ede) …………………………… 95± 25%

Itọkasi:CV ti ohun elo ELISA ko kere ju 10%.

Agbekọja Oṣuwọn

Furaltadone metabolite (AMOZ) ………………………………………………….…………100%

Furazolidone metabolite (AMOZ) …………………………………………...………………………….<0.1%

Nitrofurantoin metabolite (AHD).…………………<0.1%

Nitrofurazone metabolite (SEM) ………………………………………………………….…<0.1%

Furaltadone ………………………………………………………………………….…….11.1%

Furazolidone …………………………………………………………………………........ <0.1%

Nitrofurantoin……………………………………………………………………………………………….

Nitrofurazone ………………………………………………………….………………………….<1%

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