umkhiqizo

Mayelana

Le kit ingasetshenziswa ekuhlaziyweni komthamo kanye nekhwalithi yensalela ye-AOZ ezicutshini zezilwane (inkukhu, izinkomo, ingulube, njll), ubisi, uju namaqanda.

Ukuhlaziywa kwezinsalela zezidakamizwa ze-nitrofuran kudinga ukusekelwe ekutholeni ama-metabolites aboshwe ngezicubu zezidakamizwa zomzali we-nitrofuran, okuhlanganisa i-Furazolidone metabolite (AOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) ne-Nitrofurazone metabolite (SEM).

Uma kuqhathaniswa nezindlela zechromatographic, ikhithi yethu ibonisa izinzuzo ezinkulu mayelana nokuzwela, umkhawulo wokutholwa, okokusebenza kobuchwepheshe kanye nemfuneko yesikhathi.

Izingxenye Zekhithi

• Ipuleti le-Microtiter elihlanganiswe ne-antigen, imithombo engama-96

• Izixazululo ezijwayelekile(amabhodlela angu-6,1ml/ibhodlela)

0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb

• Ukulawula okujwayelekile kwe-spiking : (1ml/ibhodlela)..............................................….100ppb

• I-enzyme conjugate concentrate 1.5ml.............................................….….ikepisi elibomvu

• Isixazululo se-antibody sigxiliswe ku-0.8ml………………………………....…ikepisi eliluhlaza

• I-Substrate A 7ml…………………..........................................……………..ikepisi elimhlophe

• I-Substrate B7ml…………………………………...............................................…..ikepisi elibomvu

• Isixazululo sokumisa 7ml………………………………………………….………ikepisi eliphuzi

• 20×isixazululo sokugeza esigxilile esingu-40ml ………………………….……ikepisi esobala

• 2×isixazululo sokukhipha okugxilisiwe 60ml……………………..…………….ikepisi eliluhlaza okwesibhakabhaka

• 2-Nitrobenzaldehyde 15.1mg……………………………………………………ikepisi elimnyama

Ukuzwela, ukunemba nokunemba

Ukuzwela: 0.025ppb

Umkhawulo wokutholwa………………………………………………………………….1ppb

Ukunemba:

Izicubu zezilwane (imisipha nesibindi)……………………75±15%

Uju……………………………………………………..90±20%

Iqanda……………………………………………………..…90±20%

Ubisi………………………………………………………..…90±10%

Ukunemba:I-CV yekhithi ye-ELISA ingaphansi kwe-10%.

I-Cross Rate

I-Furazolidone metabolite (AOZ)……………………………………………..100%

I-Furaltadone metabolite (AMOZ)…………………………………………<0.1%

I-Nitrofurantoin metabolite (AHD)………………………………………<0.1%

I-Nitrofurazone metabolite (SEM)…………………………………………<0.1%

I-Furazolidone………………………………………………………….…..…16.3%

Furaltadone…………………………………………………………….…<1%

I-Nitrofurantoin………………………………………………………….…<1%

I-Nitrofurazone……………………………………………………………..…<1%