pwodwi

Konsènan

Twous sa a ka itilize nan analiz quantitative ak kalitatif nan rezidi AOZ nan tisi bèt (poul, bèf, kochon, elatriye), lèt, siwo myèl ak ze.

Analiz de rezidi dwòg nitrofuran bezwen baze sou deteksyon metabolit tisi mare nan dwòg paran nitrofuran, ki gen ladan metabolit Furazolidone (AOZ), metabolit Furaltadone (AMOZ), metabolit Nitrofurantoin (AHD) ak metabolit Nitrofurazone (SEM).

Konpare ak metòd kwomatografik, twous nou an montre avantaj konsiderab konsènan sansiblite, limit deteksyon, ekipman teknik ak kondisyon tan.

Konpozan Twous

• Plak mikrotiter ki kouvri ak antijèn, 96 pwi

• Solisyon estanda (6 boutèy, 1ml / boutèy)

0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb

• Spiking estanda kontwòl: (1ml / boutèy)...................................................….100ppb

• Anzim konjige konsantre 1.5ml.............................................…….bouchon wouj

• Solisyon antikò konsantre 0.8ml……………………………....…bouchon vèt

• Substra A 7ml...............................................................…..…..bouchon blan

• Substra B7ml……………………….......................................................…..…..bouchon wouj

• Stop solisyon 7ml………………………………………….………bouchon jòn

• 20×konsantre solisyon lave 40ml ……………………….……bouchon transparan

• 2×konsantre solisyon ekstraksyon 60ml…………………..…………….bouchon ble

• 2-Nitrobenzaldehyde 15.1mg……………………………………………bouchon nwa

Sansiblite, presizyon ak presizyon

Sansiblite: 0.025ppb

Limit deteksyon……………………………………… 0.1ppb

Presizyon:

Tisi bèt (misk ak fwa)……….…………75±15%

Siwo myèl………………………………………..……..90±20%

Ze……………………………………………………..…90±20%

Lèt……………………………………………………..…90±10%

Presizyon:CV nan twous ELISA a se mwens pase 10%.

Kwa Pousantaj

Furazolidone metabolit (AOZ)……………………………………………………..100%

Furaltadone metabolit (AMOZ)………………………………………<0.1%

Nitrofurantoin metabolit (AHD)………………………………………<0.1%

Nitrofurazone metabolit (SEM)………………………………………..<0.1%

Furazolidone…………………………………………………….………….…..…16.3%

Furaltadone………………………………………………………………………………<1%

Nitrofurantoin……………………………………………………….…….…<1%

Nitrofurazon………………………………………………………………..…<1%